Fig. 5

ATP enhanced BDNF synthesis and release in BV2 microglia cells. BV2 cells were treated with 50 μM ATP to activate P2X4Rs. At different time points, supernatants and cell lysates were collected. a Representative Western blot results of intracellular BDNF in BV2 cells. b The histogram presents the quantitative analysis of BDNF protein. n = 6/group, one-way ANOVA and Tukey’s multiple comparison test. *p < 0.05, ***p < 0.001 versus PBS. c Concentration of BDNF in the supernatants incubated with ATP at different time points. n = 8/group, T test. *p < 0.05, ***p < 0.001 versus PBS. d Immunofluorescence staining for BDNF (red) and Iba1 (green) at 120 min after PBS or ATP stimulation. Scale bar = 20 μm