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Fig. 2 | The Journal of Headache and Pain

Fig. 2

From: Microglia P2X4R-BDNF signalling contributes to central sensitization in a recurrent nitroglycerin-induced chronic migraine model

Fig. 2

Expression profile of the P2X4R in the TNC after the recurrent intermittent administration of NTG. a, b Western blot assay for the expression profile of P2X4Rs in VEH and NTG mice on days 3, 7, 9, and 11. One-way ANOVA and Tukey’s multiple comparison test. n = 6/group, ***p < 0.001 versus VEH. c The white dotted line frame indicates the TNC regions. d Representative photographs of immunofluorescence staining for P2X4R (green) expression in microglia (Iba-1, red) in the TNC in the VEH and NTG groups on day 11. Scale bar = 50 μm. e The white box was amplified. Scale bar = 20 μm

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