Fig. 3

Periorbital pressure and paw withdrawal thresholds after the administration of PP2 and genistein. a Periorbital pressure thresholds of the different groups. Compared with the sham group, the CM group showed a significant decrease in the periorbital pressure thresholds, but no significant difference in the von Frey test was found between the CM and CM + DMSO groups. Compared with that found for the CM + DMSO group, the administration of PP2 and genistein significantly increased the periorbital pressure thresholds. There was no significant difference in the von Frey test results among the CM + PP2 (7.3 nmol), genistein (100 ng/g) and CM + DMSO groups. The pain thresholds of the periorbital region obtained in the CM + PP2 (73 nmol) and genistein (300 ng/g) groups were significantly increased compared with those in the CM + DMSO group. b Paw withdrawal thresholds of the different groups. Compared with the sham group, the CM group showed a significant decrease in the paw withdrawal thresholds, but there was no significant difference in the von Frey test results between the CM and CM + DMSO groups. Compared with that found for the CM + DMSO group, the administration of PP2 and genistein significantly increased the periorbital pressure thresholds. There was no significant difference in the von Frey test results among the CM + PP2 (7.3 nmol), genistein (100 ng/g) and CM + DMSO groups, but there were significant differences in the von Frey test results mong the CM + PP2 (73 nmol), genistein (300 ng/g) and CM + DMSO groups (n = 10, each group, *P < 0.01 compared with the sham group, ^#P < 0.01 compared with the CM + DMSO group)