Fig. 4

HBO suppresses astrocyte activation. On postoperative day 7, sections of the DRG, spinal cord, and hippocampal tissues were stained with anti-glial fibrillary acidic protein (GFAP) (green) antibody. Nuclei were counterstained with DAPI. a Representative micrographs. b Quantification of the percentage of GFAP-labeled astrocytes. c TNF-α generation was examined by ELISA. N = 4 for each group. *P < 0.05