Fig. 6
Effect of sulforaphane pretreatment on the number of nNOS- and c-Fos-immunoreactive cells in rat TNC 4 h after NTG injection. Panels a-d correspond to nNOS immunofluorescence staining, panels f-i correspond to c-Fos immunofluorescence staining. a,f control group. b,g NTG group. c,h SFN + NTG group. d,i the white rectangle frame indicates the representative anatomical site observed. Quantitative analysis showed that nNOS-immunoreactive e and c-Fos-immunoreactive cells j were all increased in the NTG group compared to those in the control group, but decreased in the SFN plus NTG group compared to those in the H2O plus NTG group. (* P < 0.05 for SFN + NTG vs H2O + NTG group, # P < 0.05 for NTG vs control group, n = 6 per group) (a, b, c, f, g, h: Bar = 50 μm; d, i: Bar = 200 μm)