Table 1 Overview of the experiments, test groups (n = 12), and compounds used
|  | Pre-treatment compound |  +  | Test day compound |
|---|---|---|---|
Experiment 1: Anti-PACAP mAbs + PACAP38 | |||
  Group 1 | IgG control (30 mg/kg) |  +  | Vehicle (saline) |
  Group 2 | Anti-PACAP mAbs (30 mg/kg) |  +  | Vehicle (saline) |
  Group 3 | IgG control (30 mg/kg) |  +  | PACAP38 (2 µg/kg) |
  Group 4 | Anti-PACAP mAbs (30 mg/kg) |  +  | PACAP38 (2 µg/kg) |
Experiment 2: Anti-PACAP mAbs + GTN | |||
  Group 1 | Anti-PACAP mAbs (30 mg/kg) |  +  | Vehicle (12.8% alcohol) |
  Group 2 | IgG control (30 mg/kg) |  +  | GTN (10 mg/kg) |
  Group 3 | Anti-PACAP mAbs (30 mg/kg) |  +  | GTN (10 mg/kg) |
Experiment 3: Anti-PACAP mAbs + Levcromakalim | |||
  Group 1 | Anti-PACAP mAbs (30 mg/kg) |  +  | Vehicle (2% DMSO) |
  Group 2 | IgG control (30 mg/kg) |  +  | Levcromakalim (1 mg/kg) |
  Group 3 | Anti-PACAP mAbs (30 mg/kg) |  +  | Levcromakalim (1 mg/kg) |
Experiment 4: Sumatriptan + PACAP38 | |||
  Group 1 | Vehicle (saline) |  +  | Vehicle (saline) |
  Group 2 | Vehicle (saline) |  +  | PACAP38 (2 µg/kg) |
  Group 3 | Sumatriptan (0.6 mg/kg) |  +  | PACAP38 (2 µg/kg) |