Fig. 5

Sumatriptan had no effect on PACAP38-induced hypersensitivity. Data are shown as square root transformed (SQRT) 50% withdrawal threshold (g). A Responses one hour after PACAP38 (2 µg/kg, s.c.) or vehicle injection in wildtype mice (n = 12 per group) pre-treated with sumatriptan (0.6 mg/kg, i.p.) or vehicle (i.p.) on five test days. Two-way ANOVA with Tukey’s post hoc test for multiple comparisons was used with data represented as means ± SEMs. * = P < 0.05, ** = P < 0.01, *** = P < 0.001, and **** = P < 0.0001. B Descriptive visualization of individual data points for baseline and day 9 of the curves on the left-hand side (Fig. 5A) with mean bars and SEMs