Fig. 4

Levcromakalim signaling independent from PACAP. Data are shown as 50% withdrawal threshold (g) and was square root transformed (SQRT). A Acute responses two hours after levcromakalim (1 mg/kg, i.p.) or vehicle (i.p.) injection in wildtype mice (n = 12 per group) pre-treated with anti-PACAP antibody or IgG control (30 mg/kg, i.p.) on five test days. Two-way ANOVA with Tukey’s post hoc test for multiple comparisons was used with data represented as means ± SEMs. * = P < 0.05, ** = P < 0.01, *** = P < 0.001, and **** = P < 0.0001. B Descriptive visualization of individual data points for baseline and day 9 of the curves on the left-hand side (Fig. 4A) with mean bars and SEMs